Abstract The current study attempted to discover the factors that affect students' acceptance of mobile learning in public and private Jordanian universities. Linear regression technique has been used through the self-administrated questionnaire. The study sample consisted of 620 students from Ajloun National University, Jadara University and Yarmouk University. The results of the study demonstrated the validity of the study hypotheses and that the specific factors (performance expectancy, effort expectancy, social influence, facilitating conditions influence the acceptance of mobile learning. This research contributes to identifying the factors that affect the acceptance of university students and their use of mobile learning in Jordan. According to the results of the study, the researcher recommends the university administration to pay attention to the factors that affect the students' acceptance of mobile learning to achieve the academic program outputs.