(The present study was conducted to find out effectiveness of differentiated instructions in teaching of environmental studies in primary school. The study has provided an opportunity for researcher to work with school teacher in developing differentiated instructions and used developed material in teaching learning process. A sample of 60 students were selected from third standard of Mararhi medium school through purposive sample technique from Bhivandi area. Experimental method was used in present study. The study revealed that differentiated instructions was very much beneficial for students to achieved mastery on the subject . Majority of Students having opinion that the this type of instructions created interest in the studies. The result revealed in the study indicated that learning using differentiated instructions contribute significant increase in academic achievement of students to compare to traditional method)