An investigation was made to find out whether there is any significant difference between values and gender of pupil teachers in respect of Theoretical value, Economic value. Political value, Social value, Aesthetic value. In the present study, random sampling technique was adopted to select a sample of 250 Pupil teachers in Guru Nanak College of Education Dalewal, Hoshiarpur and ‘Students Value Inventory’ which was developed and standardized by T. Padmanaban, used for collecting data from pupil teachers. The result revealed that there is no significant difference between values of male and female pupil teachers in respect of Religious value. But there is significant difference between values of male and female pupil teachers in respect of Theoretical, Economic, Political, Social and Aesthetic values.