Taking Ketone bodies metabolism as an example, this paper carried out the problem-oriented flipped classroom teaching practice research based on knowledge framework as the main line in biochemistry. The classroom teaching mode helped students to clear up the ideas quickly, grasp the key points based on the knowledge framework and problem-oriented, and then learn, think and discuss actively and deeply, improved students skills and learning initiative, so that improved students autonomouslearningenthusiasmand critical thinking ability, at the same time, helped teachers to condense, expand and deepen knowledge points continuously, improve the quality and effectiveness of problem-oriented, and then improve the teaching efficiency and quality, and thus achieve teaching others is teaching yourself.