{"references": ["SADER. Obtenido https://www.gob.mx/agricultura", "Moguel P, Toledo VM. Biodiversity conservation and traditional coffee systems of Mexico. Biol Conserv 1999; 13:11-21.", "Cerd\u00e1n CR, Rebolledo MC, Soto G, Rapidel B, Sinclair FL. Local knowledge of impacts of tree cover on ecosystem services in smallholder coffee production systems. Agric Syst 2012; 110:119-130. doi:10.1016/j.agsy.2012.03.014", "Chait G. Caf\u00e9 en Colombia: servicios ecosist\u00e9micos, conservaci\u00f3n de la biodiversidad. Sistemas agroforestales, funciones productivas, socioecon\u00f3micas y ambientales. Turrialba: CATIE 2015; 349-364.", "Villarreyna R, Avelino J, Cerda R. Adaptaci\u00f3n basada en ecosistemas: efecto de los \u00e1rboles de sombra sobre servicios ecosist\u00e9micos en cafetales. Agron Mesoam 2020; 31(2): 499-516. doi:10.15517/am.v31i2.37591", "Manson RH, Hern\u00e1ndez-Ortiz V, Gallina S, Mehltreter K. Agroecosistemas cafetaleros de Veracruz: biodiversidad, manejo y conservaci\u00f3n. Instituto de Ecolog\u00eda, A.C. e Instituto de Nacional de Ecolog\u00eda (INE-SEMARNAT): M\u00e9xico 2008.", "Heredia A, Arias RM. Hongos saprobios y endomicorriz\u00f3genos en suelos. In: Manson RH, Hern\u00e1ndez-Ortiz V, Gallina S, Mehltreter K, Eds. Agroecosistemas cafetaleros de Veracruz: biodiversidad, manejo y conservaci\u00f3n; 2008; 193-212.", "Arias RM, Heredia-Abarca G, Sosa VJ, Fuentes-Ram\u00edrez LE. Diversity and abundance of arbuscular mycorrhizal fungi spores under different coffee production systems and in a tropical montane cloud forest patch in Veracruz, Mexico. Agrofor Syst 2012; 85(1):179-193. https://doi.org/10.1007/s10457-011-9414-3", "Camargo-Ricalde SL, Monta\u00f1o NM, De la Rosa-Mera CJ, Monta\u00f1o ASA Micorrizas: una gran uni\u00f3n debajo del suelo 2012; Revista Digital Universitaria 13(7). http://www.revista.unam.mx/vol.13/num7/art72/index.html\"", "Saha R, Mondal B, Naskar B. AMF inoculation changes, the root development pattern of plants at early stage of colonization. Int J Bio-resource Environ Agric Sci 2014; 1:43-47.", "Zou YN, Srivastava AK, Wu QS. Glomalin: A potential soil conditioner for perennial fruits. Int J Agric Biol 2016; 18: 293-297.", "Martinez LB, Pugnaire FI. Interacciones entre las comunidades de hongos formadores de micorrizas arbusculares y de plantas. Algunos ejemplos en los ecosistemas semi\u00e1ridos. Ecosistemas 2009; 18(2): 44-54.", "Yao Q, Zhu HH. Arbuscular mycorrhizal fungi: A belowground regulator of plant diversity in grasslands and the hidden mechanisms. In: Runas J, Dahlgren T, Eds. Grassland Biodiversity-Habitat Types, Ecological Processes and Environmental Impacts New York: Nova Science Publisher; 2010;1-14.", "Smith S, Read DA. Mycorrhizal symbiosis, Academic Press 2010.", "Fisher JB, Jayachandran DK. Arbuscular mycorrhizal fungi enchance seedling growth in two endangered plant species from South Florida. Int J Plant Sci 2002; 163:559-566. https://doi.org/10.1086/340428.", "Six J, Bossuyt H, Degryze S, Denef K. A history of research on the link between (micro) aggregates, soil biota and soil organic matter dynamics. Soil Tillage Res 2004; 79:7-31. https://doi.org/10.1016/j.still.2004.03.008.", "Gao W, Wang P, Wu Q-S. Functions and application of glomalin-related soil proteins: a review. Sains Malays 2019; 48(1): 111\u20139. DOI:10.17576/jsm-2019-4801-13", "Lozano S\u00e1nchez JD, Armbrecht I, Montoya Lerma J. Hongos formadores de micorrizas arbusculares y su efecto sobre la estructura de los suelos en fincas con manejos agroecol\u00f3gicos e intensivos. Acta Agron 2015; 64(4): 289-296.", "Wright S, Upadhyaya A. Extraction of an abundant and unusual protein from soil and comparison with hyphal protein of arbuscular mycorrhizal fungi. Soil Sci 1996; 161: 575-586.", "Ryan MG, Graham JH. Is there a role for arbuscular mycorrhizal fungi in production agriculture?. Plant Soil 2002; 244: 263-271. https://doi.org/10.1023/A:1020207631893.", "Morell F, Hern\u00e1ndez A, Borges Y, Marentes FL. La actividad de los hongos micorr\u00edzicos arbusculares en la estructura del suelo. Cult Trop 2009; 30(4):00-00.", "Rillig MC, Wright SF, Nichols KA, Schmidt WF, Torn MS. Large contribution of arbuscular mycorrhizal fungi to soil carbon pools in tropical forest soils. Plant Soil 2001; 233:167-177. https://doi.org/10.1023/A:1010364221169.", "Rillig MC, Maestre FT, Lamit LJ. Microsite differences in fungal hyphal length, glomalin, and soil aggregate stability in semiarid Mediterranean steppes. Soil Biol Biochem 2003; 35:1257-1260. https://doi.org/10.1016/S0038-0717(03)00185-8", "Cornejo P, Meier S, Borie G, Rillig MC, Borie F. Glomalin-related soil protein in a Mediterranean ecosystem affected by a copper smelter and its contribution to Cu and Zn sequestration. Sci Total Environ 2008; 15: 406(1-2): 154-60. doi: 10.1016/j.scitotenv.2008.07.045.", "Gerdemann JW, Nicolson TH. Spore of mycorrhizal endogone species extracted from soil by wet sieving and decanting. Trans Br Mycol Soc 1963; 46: 234-44.", "Wrigth SF, Upadhyaya A. A survey of soils for aggregate stability and glomalin, a glycoprotein produced by hyphae of arbuscular mycorrhizal fungi. Plant and Soil 1998; 198(1): 97-107.", "Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72(1-2): 248-54.", "StatSoft, Inc. Statistica para Windows v. 10.0. Data analysis software system. Tulsa. [cd-Rom] 2017.", "NORMA Oficial Mexicana NOM-021-SSA1-2021, Salud ambiental. Criterio para evaluar la calidad del aire ambiente, con respecto al mon\u00f3xido de carbono (CO). Valores normados para la concentraci\u00f3n de mon\u00f3xido de carbono (CO) en el aire ambiente, como medida de protecci\u00f3n a la salud de la poblaci\u00f3n.", "Cogo FD, Saggin J\u00fanior OJ, Guimar\u00e3es PTG, Siqueira JO, Carneiro MAC. High rates of agricultural gypsum affect the arbuscular mycorrhiza fungal community and coffee yield. Bragantia 2020; 79: 612-622.", "Rillig MC, Wright SF, Shaw MR, Field CB. Artificial climate warming positively affects arbuscular mycorrhizae but decreases soil aggregate water stability in an annual grassland. Oikos 2002; 97: 52-58. https://doi.org/10.1034/j.1600-0706.2002.970105.x.", "Lutgen ER, Muir-Clairmont D, Graham J, Rillig MC. Seasonality of arbuscular mycorrhizal hyphae and glomalin in a western Montana grassland. Plant and Soil 2003; 257(1): 71-83. https://doi.org/10.1023/A:1026224209597.", "Nichols KA, Wright SF. Comparison of glomalin and humic acid in eight native US soils. Soil Sci 2005; 170: 985-997. doi: 10.1097/01.ss.0000198618.06975.3c.", "Batten KM, Six J, Scow KM, Rillig MC. Plant invasion of native grassland on serpentine soils has no major effects upon selected physical and biological properties. Soil Biol Biochem 2005; 37(12): 2277-2282. https://doi.org/10.1016/j.soilbio.2005.04.005", "Treseder KK, Turner KM. Glomalin in ecosystems. Soil Sci Soc Am J 2007; 71: 1257-1266. https://doi.org/10.2136/sssaj2006.0377.", "Wright SF, Anderson RL. Aggregate stability and glomalin in alternative crop rotations for the central Great Plains. Soil Biol Biochem 2000; 31: 249-253. https://doi.org/10.1007/s003740050653", "Knorr MA, Boerner REJ, Rillig MC. Glomalin content of forest soils in relation to fire frequency and landscape position. Mycorrhiza 2003; 13: 205-210. https://doi.org/10.1007/s00572-002-0218-1.", "Steinberg PD, Rillig MC. Differential decomposition of arbuscular mycorrhizal fungal hyphae and glomalin. Soil Biol Biochem 2003; 35: 191-194. https://doi.org/10.1016/S0038-0717(02)00249-3", "Rillig MC, Wright SF, Kimball BA, Pinter PJ, Wall GW, Ottman MJ, Leavitt SW. Elevated carbon dioxide and irrigation effects on water stable aggregates in a sorghum field: A possible role for arbuscular mycorrhizal fungi. Glob Change Biol 2001; 7: 333-337. https://doi.org/10.1046/j.1365-2486.2001.00404.x.", "Lovelock CE, Wright SF, Nichols KA. Using glomalin as an indicator for arbuscular mycorrhizal hyphal growth: An example from a tropical rain forest soil. Soil Biol Biochem 2004; 36: 1009-1012. https://doi.org/10.1016/j.soilbio.2004.02.010.", "Rodr\u00edguez-Yon Y, Chiriboga-Morocho R, Concha-Egas TG, de Le\u00f3n-Lima DP. Caracterizaci\u00f3n de las fracciones de glomalina en suelos Ferral\u00edticos Rojos con diferente uso. Cultivos Tropicales 2020; 41(4):e04", "Gonz\u00e1lez\u2013Ch\u00e1vez MC, Guti\u00e9rrez MC, Wright S. (2004). Hongos micorr\u00edzico arbusculares en la agregaci\u00f3n del suelo y su estabilidad, Terra Latinoam 2004; 22: 507-214.", "Purin S, Rillig MC. The arbuscular mycorrhizal fungal protein glomalin: Limitations, progress, and a new hypothesis for its function. Pedobiologia 2007; 51(2): 123-130. doi:10.1016/j.pedobi.2007.03.002", "Wright SF, Upadhyaya A. Quantification of arbuscular mycorrhizal fungi activity by the glomalin concentration on hyphal traps. Mycorrhiza 1999; 8(5): 283-285.", "Prasad M, Chaudhary M, Ramakrishnan S, Mahawer SK. Glomalin: a miracle protein for soil sustainability. Indian Farmer 2018; 5(9): 1092\u2013100.", "Gomathy M, Sabarinathan KG, Sivasankari-Devi T, Pandiyarajan P. Arbuscular mycorrhizal fungi and glomalin-super glue. Int J Curr Microbiol Appl Sci 2018; 7(7): 2853\u20137. DOI:10.20546/ijcmas.2018.707.334", "De la Cruz-Elizondo, Y, Fontalvo-Buelvas JC. Biolog\u00eda del suelo. C\u00d3DICE/ Taller Editorial/Suma Textual: M\u00e9xico 2019.", "Gallardo LJF. La materia org\u00e1nica del suelo. Residuos org\u00e1nicos, humus, compostaje y captura de carbono. Sociedad Iberiamericana de F\u00edsica y Qu\u00edmica Ambiental. J\u00e1ser Proyectos Editoriales/Nueva Graficesa: Espa\u00f1a 2016.", "Zhong Z, Wang W, Wang Q, Wu Y, Wang H, Pei Z. Glomalin amount and compositional variation, and their associations with soil properties in farmland, northeastern China. J Plant Nutr Soil Sci 2017; 180:563-575. DOI: 10.1002/jpln.201600579.", "B\u00e1ez-P\u00e9rez A, Gonz\u00e1lez-Ch\u00e1vez MC, Etchevers-Barra JD, Prat C, Hidalgo-Moreno C. Glomalin and carbon sequestration in cultivated tepetates. Agrociencia 2010; 44(5): 517\u201329.", "Bird SB, Herrick JE, Wander MM, Wright SF. Spatial heterogeneity of aggregate stability and soil carbon in semi\u2013arid rangeland. Environ Poll 2002; 116: 445\u2013455. DOI: 10.1016/s0269-7491(01)00222-6", "Rillig MC, Ramsey PW, Morris S, Paul EA. Glomalin, an arbuscular\u2013mycorrhizal fungal soil protein, responds to land\u2013use change. Plant Soil 2003; 253:293-299. https://doi.org/10.1023/A:1024807820579.", "Morales A, Castillo CG, Rubio R, Godoy R, Rouanet JL, Borie F. Niveles de glomalina en suelos de dos ecosistemas del sur de Chile. RC Suelo Nutr Veg 2005; 5(1): 37-45. http://dx.doi.org/10.4067/S0717-92002008000100002", "Holtz EWF, Giuffr\u00e9 L, Ciarlo E, Cortinez AG. Glomalin and Its relationship with inoculation, fertilization and soils with different sand proportion. J Agric Ext Rural Dev 2018; 11(2): 24-32. DOI:10.9790/2380-1102022432"]}

RESUMEN Antecedentes: La estructura vegetal de los cafetales con sombra ha funcionado como nicho de la biota nativa. Entre esta biota destacan los hongos micorrízicos arbusculares que forman simbiosis con las plantas y liberan una glicoproteína llamada glomalina que contribuye a la reserva de carbono en los suelos. Objetivo: Caracterizar los niveles de las fracciones de proteínas del suelo relacionadas a glomalina total y fácilmente extraíble y analizar su relación con el número de esporas de los hongos micorrízicos arbusculares y con algunas características fisicoquímicas de los suelos cafetaleros. Métodos: Se analizaron suelos de cinco cafetales bajo sombra y se utilizaron diferentes métodos extractivos, seguido de la estimación de la concentración de proteínas para la determinación de glomalina y un conteo de esporas de los hongos micorrízicos arbusculares. Resultados y discusión: Los valores de glomalina fácilmente extraíble y total oscilaron entre 0.15-a 0.46 y 0.57-2.03 mg/kg respectivamente. La finca Jilotepec1 presentó los mayores valores de las dos fracciones de glomalina. Las regresiones lineales revelaron una relación significativa positiva entre la glomalina total con la materia orgánica, con el carbono orgánico, con el nitrógeno y carbono total. Se observó una relación significativa negativa de la glomalina total con el fósforo disponible y la densidad aparente del suelo. No obstante que no se observó una relación significativa entre la glomalina con el número de esporas, se denota una tendencia, de tal manera que es factible utilizar la medición de glomalina para cuantificar la actividad de los hongos micorrízicos arbusculares en las fincas cafetaleras. ABSTRACT Background: The vegetal structure of coffee plantation under shadow has functioned as niche of native biota. Among this biota, its highlight arbuscular mycorrhizal fungi that form symbiosis with these plants and release a glycoprotein named glomalin which contributes to the carbon reserve in soils. Objective: To characterize the levels of the fractions of glomalin-related soil proteins and easy extractable and their relationship with the number of arbuscular mycorrhizal fungi spores and several physic-chemical characteristics of coffee soils. Methods: Soils of five coffee plantations under shadow with the same management were analyzed. Different extractive methods were used, followed by the protein-concentration estimation to determine glomalin and the arbuscular mycorrhizal fungi spores counting was achieved. Results and discussion: Easily extractable and total glomalin values ranged from 0.15-0.46 and 0.57-2.03 mg/kg, respectively. The Jilotepec1 coffee plantation presented the highest values of the two glomalin fractions. Linear regressions revealed a significant positive relationship between total glomalin with organic matter, organic carbon, and total nitrogen and carbon. A significant negative relationship of total glomalin with soil available phosphorus and soil bulk density. Although a significant relationship between glomalin and the number of spores was not observed, a trend is denoted, in such a way that glomalin can be used to quantify the activity of arbuscular mycorrhizal fungi in coffee plantations.