Chromatography is a vital biophysical method that allows for the separation, identification, and filtering of components in order to conduct quantitative research. High efficiency, quicker separation speed, high flow rate, increased detection sensitivity, and cost savings are all advantages of novel chromatography methods. The goal of using chromatography, which is a technique for quantitative investigation separated from its partition, is to accomplish a satisfactory separation in a reasonable amount of time. The objective of chromatography is to analyze a sample qualitatively and quantitatively and its primary function is to clean and separate at least one component of the sample. It examines new and emerging chromatography techniques, as well as main standards. Phospholipids can be determined qualitatively and quantitatively using a variety of analytical procedures. Current new chromatographic methods are presented in this review.