This is the dataset accompanying the submission of the manuscript: Label-free detection of methicillin resistance in Staphylococcus aureus using different Raman-spectroscopy approaches in the journal Microbiology Spectrum. The data description is the following: Strains 16859MRSA= Strain AUSTR-07-16859 MRSA 16859MSSA= Strain AUSTR-07-16859 MSSA CC8MRSA= Strain 08V15773 CC8MSSA= Strain MRSA2010-174 AUSTR05MRSA= Strain AUSTR-05-15441 MRSA AUSTR05MSSA= Strain AUSTR-05-15441 MSSA CC361MRSA= Strain UAE-Abu Dhabi-020 CC361MSSA= Strain UAE-Dubai-80-MS 1368.9/09 Datasets UVRR: UV-Resonance Raman with 244 nm excitation on bulk samples, calibration standard Polystyrene, measurements were time series of 10 consecutive spectra, for each strain and batch 25 time series were collected from 3 different slides 532nm: Single cell analysis with 532nm excitation, calibration standard 4AAP, one spectrum per bacterial cell was collected 785nm: Bulk analysis of bacterial colonies using 785 nm excitation and a Raman fibre probe, calibration standard 4AAP, bulk analysis, individual spectra of colonies were collected Data structure is in the metadata files. Individual spectra are in the folders sorted by the date they were measured.