DESCRIPTION OF GORDONIA MANGROVI SP. NOV. Gordonia mangrovi (man.gro ˊ vi. N.L. gen. n. mangrovi of a mangrove, referring to the isolation of the type strain from mangrove soil). Cells are Gram-stain-positive, aerobic and rod-shaped. Colonies grow well on ISP2 agar and are reddish-orange. Grows on ISP1, ISP3, ISP5 and ISP7 agar, but not on ISP4 or ISP6 agar. Diffusible pigments including melanoid pigments are not formed. Growth occurs at pH 5–10, at 20–40 °C and in the presence of 0–8% NaCl. Catalase activity and nitrate reduction are positive. Degrades arbutin, but not aesculin, casein, hypoxanthine or xanthine. D-Arabitol,D-fructose, D-glucose,D-mannose, trehalose,L-rhamnose, meso -inositol and sucrose are utilized as sole carbon sources, but not cellulose, cellobiose, D-galactose, lactose,D-xylose or raffinose. L-Arginine,L-asparagine,L-cysteine, L-methionine, L-proline, L-serine, L-threonine and L-valine are used as sole nitrogen sources, but not L-histidine orL-phenylalanine. Resistant to chloramphenicol, gentamicin, kanamycin, nalidixic acid, novobiocin, penicillin G, sulfamethoxazole, tetracycline and tobramycin, but sensitive to rifampin. The cell wall contains meso -diaminopimelic acid. The wholecell sugars are arabinose and galactose. Mycolic acids are present. The menaquinones are MK-9(H 2) an MK-7(H 2). The predominant phospholipids are composed of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major fatty acids are C 16:0, C 16:1 ω 7 c /C 16:1 ω 6 c, C 18:0 10-methyl (TBSA), C 18:0 and C 18:1 ω 9 c. The DNA G+C content of the type strain is 67.0mol%. The type strain, HNM0687 T (=CCTCC AA 2019074 T =KCTC 49383 T), was isolated from mangrove soil. The GenBank accession number for the 16S rRNA gene of strain HNM0687 T is MN653213. The GenBank accession number for the gyrB gene of strain HNM0687 T is MN759309. The GenBank accession number for the genome sequence of strain HNM0687 T is WMBR00000000.

Published as part of Xie, Yuhui, Zhou, Shuangqing, Xu, Yun, Wu, Wenqiang, Xia, Wei, Zhang, Rongping, Huang, Dongyi & Huang, Xiaolong, 2020, Gordonia mangrovi sp. nov., a novel actinobacterium isolated from mangrove soil in Hainan, pp. 4537-4543 in International Journal of Systematic and Evolutionary Microbiology 70 (8) on page 4541, DOI: 10.1099/ijsem.0.004310, http://zenodo.org/record/6223959