Phyzelaphryne nimio sp. nov. Figures 5–10, 12 Holotype. MCP 13719 (field code SCF 1846). An adult female, collected by G. Gagliardi-Urrutia, F.J.M. Rojas- Runjaic, P.I. Simões and S. Castroviejo-Fisher on 5 February 2017, in an area of terra-firme forest on the west bank of the Juami River (Canal da Inveja), within Estação Ecológica Juami-Japurá, state of Amazonas, Brazil (1.75834° S, 67.61532° W; ~ 67 m a.s.l.). Paratypes. Thirty-three adult specimens, 16 females and 18 males. Females: MCP 13683–13697, 13720 (field codes SCF 1877, 2003, 1968, 1969, 1967, 1973, 1907, 1775, 1972, 1970, 1908, 1875, 1820, 1743, 1847, 1971, respectively). Males: MCP 13698–13715 (field codes SCF 1910, 1959, 1909, 1964, 1974, 1965, 1912, 1744, 1745, 1961, 1962, 1960, 1849, 1966, 1876, 1848, 1742, 1911, respectively). All collected by G. Gagliardi-Urrutia, F.J.M. Rojas-Runjaic, P.I. Simões and S. Castroviejo-Fisher between 2–9 February 2017 at the same site as the holotype and at a second sampling site within EEJJ (Igarapé da Fartura), on the east bank of the middle course of the Juami River (1.96063° S, 67.93694° W, ~ 71 m a.s.l.). Referred specimens. MCP 13716, 13717 and 13718 (field codes SCF 1913, 1963 and 1978 respectively), all juvenile specimens, collected at Igarapé da Fartura sampling site, between 6–8 February 2017, same collectors as the paratypes. MCP 13725 (field code SCF 1975), three eggs laid by female paratype MCP 13688 on 0 8 February 2017. Etymology. The specific epithet nimio is a Spanish masculine adjective derived from the Latin word nimius (“abundant” or “plentiful”). The Spanish term keeps this meaning but has also received the additional meaning of “insignificant” and “very small” (Real Academia Española 2014). The dual meaning of nimio alludes simultaneously to the abundance of the new species in the two localities where it was collected and to its very small body size, and is used in apposition to the genus. Generic placement. We assign this new species to Phyzelaphryne based on its phylogenetic position using molecular data, and on the presence of the following diagnostic phenotypic characters, proposed by Hoogmoed & Lescure (1984): (1) Digits round in cross section; (2) discs on fingers and toes small, not expanded to moderately expanded, pointed at their tips; (3) discs of fingers III and IV and discs of toes I–V with distinct lateral grooves, interrupted only at the tip; (4) number of phalanges in fingers I–IV: 2-2-3-3; (5) number of phalanges in toes I–V: 2-2-3-4-3; (6) terminal phalanges in most fingers and toes with T-shaped tips; (7) tongue with a narrow anterior stem, widening into a large subcircular shape posteriorly; (8) a well-defined oblique subtympanic glandular ridge, not in contact with the tympanic annulus; (9) inguinal black spot absent. Definition. Phyzelaphryne nimio is characterized by: (1) small body size, males 11.2 to 15.2 mm and females 13.2 to 15.9 mm in SVL; (2) skin on dorsum shagreened; ventral surfaces smooth, finely tuberculate only on thighs; (3) snout sub-acuminate in dorsal view, protruding in lateral view; (4) tympanum round and small, horizontal diameter approximately 30 % of eye diameter; tympanic annulus concealed dorsally by a skin fold; (5) subtympanic glandular ridge present from the mouth rictus to approximately the insertion of upper arm; (6) two or three pentagonal, trapezoid or ellipsoid supernumerary tubercles of which the one at the base of finger III is much larger than the others; (7) when conspicuous, supernumerary tubercle at the base of finger IV is ellipsoid, fused laterally with tubercle at the base of finger III; (8) thenar tubercle fused with subarticular tubercle on finger I; (9) inner metatarsal tubercle fused with subarticular tubercle of toe I; (10) cryptic body coloration, background color of dorsum tan or dark brown with darker brown mottling and small light brown or white spots, flanks tan or dark brown with white or light cream spots, ventral surfaces of body and limbs grayish brown, darkest on chest, with white blotches or spots; (11) iris golden copper with black reticulations and a bright red pupil ring. Description of holotype. Adult female (SVL = 15.3 mm) in good state of preservation, with a piece of muscle ventrally cut from the right thigh and preserved as tissue sample (Fig. 5). Measurements of the holotype are in Table 1. Body robust, head wider than long (HL/HW = 0.9), head length 0.3 times the SVL. Eye length greater than distance from anterior corner of eye to nostril (EN/EL = 0.7). Nares located posterolaterally to tip of snout, directed laterally. Nares opening laterally, visible in lateral view, but not distinct in ventral or dorsal views. Internasal distance 0.3 times HW. Snout sub-acuminate in dorsal view, protruding in lateral view. Canthus rostralis distinct, sharply angular from anterior corner of the eye to nostril, rounded from nostril to tip of snout. Loreal region slightly concave. Interorbital distance only slightly greater than internasal distance (IO/IN = 1.1). Distance between orbits 1.1 times the maximum width of eyelids, when measured dorsally (Fig. 5). Tympanum round, 0.3 times maximum length of eye. Tympanum distinct to the naked eye, tympanic annulus present, well-defined, one fourth of right tympanum concealed posterodorsally (Fig. 6), one third of left tympanum concealed dorsally. Tympanum separated from posterior corner of the eye by a distance of 0.5 times the maximum horizontal diameter of tympanum. Subtympanic glandular ridge present from the mouth rictus to approximately the insertion of the upper arm. Subtympanic glandular ridge not contacting the tympanic annulus. Anterior 25 % of tongue attached to the mouth floor, forming a narrow stem. Tongue widening into a subcircular shape posteriorly. Choanae round, positioned anteriorly to prevomerine processes and eye bulge. Dentigerous processes straight, not in contact, sharply separated, forming a transverse row, located between the choanae and the eye bulge. Vomerine teeth present, not visible under maximum (60 X) magnification but detectable by moving a wire probe along the vomerine surface. Lengths of upper arm and forearm 0.2 and 0.3 times the SVL. Palmar tubercle round to slightly elliptical. Thenar tubercle present, oval, slightly pointed distally, fused with subarticular tubercle of finger I. Maximum diameter of thenar tubercle 62 % of maximum diameter of palmar tubercle (Fig. 7). Three supernumerary tubercles present at the bases of fingers II, III and IV. Supernumerary tubercle at the base of finger III pentagonal, about the same diameter as palmar tubercle, 1.3 times size of pentagonal supernumerary tubercle at the base of finger II. Supernumerary tubercle at the base of finger IV small, ellipsoid, flat and fused laterally with supernumerary tubercle at the base of finger III. Subarticular tubercles on fingers I to IV one, one, two and one respectively. Subarticular tubercles generally round, protuberant, slightly exceeding the width of phalanges. Subarticular tubercle on finger I elliptical, distally acuminate. Fingers unwebbed. Tip of Finger IV reaching the center of distal subarticular tubercle of Finger III when fingers are juxtaposed. Relative lengths of fingers: IV

Published as part of Simões, Pedro Ivo, Costa, João Carlos Lopes, Rojas-Runjaic, Fernando J. M., Gagliardi-Urrutia, Giussepe, Sturaro, Marcelo José, Peloso, Pedro L. V. & Castroviejo-Fisher, Santiago, 2018, A new species of Phyzelaphryne Heyer, 1977 (Anura: Eleutherodactylidae) from the Japurá River basin, with a discussion of the diversity and distribution of the genus, pp. 203-230 in Zootaxa 4532 (2) on pages 216-224, DOI: 10.11646/zootaxa.4532.2.2, http://zenodo.org/record/2615218