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Heat shock proteins (HSP) are a family of stress-responsive proteins, which has important role in cell protection from different stressors. Cows suffer from metabolic stress during early lactation, which is characterized with lipolysis increased and ketogenesis. The aim of this study is to measure HSP70 concentration in blood during periparturient period, and examine relationship between HSP70 concentration, lipolysis and ketogenesis. The experiment included 20 Holstein-Friesian cows. Blood samples were taken by venepunction of v.coccigea at week before calving and 1, 2, 4 and 8 weeks after calving. Concentration of HSP70 in blood is significantly higher in early lactation in comparison with week before calving. Also, higher concentration of NEFA and BHB were found in first and second weeks after lactation. HSP70 are in positive correlation with NEFA and BHB. Partially, correlations in first and second week after calving were stronger (when there is higher lipid mobilization and ketogenesis) then overall correlation, but it loses in fourth and eight weeks after calving. We can conclude that increases HSP70 concentration in first two weeks after calving is depend of level of lipid mobilization and ketogenesis. Metabolic adaptation as lipid mobilisation and ketogenesis upregulates HSP70 in dairy cows during early lactation.