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The objective of this study was to examine the effect of plant growth promoting bacteria Pseudomonas sp., Bacillus sp., and Streptomyces sp. on germination and growth of English ryegrass and microbiological activity in its rhizosphere. One hundred seeds of perennial ryegrass inoculated with the appropriate bacteria were placed on filter paper in the dark at 22 C° until germination. Five and fourteen days after, hypocotyl and root length of seedlings were measured. The experiment was conducted in 10 l volume vegetation pots, with the following variants: 1. Pseudomonas sp. P12, 2. Bacillus sp. B1, 3. Streptomyces sp. A3, 4. control - no inoculation. Thirty and sixty days after sowing, length of stem and roots of plants (cm) were determined. Thirty days after sowing, the number of microorganisms was determined, using the dilution method. Application of Pseudomonas sp. P12 and Bacillus sp. B1 had a negative effect on ryegrass seed germination, while the use of Streptomyces sp. A3 gave positive results. On average, the application of Pseudomonas sp. P12 and Bacillus sp. B1 had the greatest effect on the stem and root length of English ryegrass. The number of the investigated groups of microorganisms increased in all variants in comparison to the control. The best effect was achieved with Bacillus sp. B1.