Freshwater bodies are one of the most common and stable habitats of biosphere. The freshwater habitats have their own physico-chemical and biological characters which are subjected to modify by local conditions and physiographic features. The water quality parameters have a great influence on the growth and other factors of aquatic organisms. Therefore, the lentic water body gives a good source for fisheries. The present investigation deals with limnological and physico-chemical parameters and their influence on Ichthyofauna abundance in Manakondur fresh water Lake at Karimnagar District, Telangana State. The study was carried out for a period of one year i.e., from June, 2020 to May, 2021.The investigation was focused on the determination of hydro-chemical parameters such as Water temperature(19.0-31.0ºC),Transparency(18.50-44.30cm),TDS(200-350mg/l),PH(7.5-8.3),DO(5.2-12.0mg/l),Co2 (3.0-9.2mg/l),TH(110-210mg/l),TA(165-300mg/l),CL(35.00-50.20mg/l),Po4 (0.02-0.16mg/l),No3 (0.02-0.14mg/l) and BOD(2.5-7.0mg/l).The study was made to recorded fish fauna available. Total 33 species of fishes were collected and identified during the study period which belongs to 6 orders, 12 families and 18 genera. The order Cypriniformes was dominant with 15 species, followed by Siluriformes (8 species), Osteoglosiformes(2 species), Perciformes(4 species), Channiformes(3 species), Perciformes(4 species) and Antherniformes(1 species) were identified. Order wise percentage composition is Cypriniformes (17%), Siluroformes (34%), (Osteoglossiformes(8%), Perciformes (25%), Channiformes (8%), Anthrniformes (8%) In the light of recent literature, the data has been discussed and it is concluded that limnological and physico-chemical parameters in this reservoir are most comply with suitability of human consumption and favourable for fishery. In the light of recent literature, the data has been discussed and it is concluded that limnological and physico-chemical parameters in this reservoir are most comply with suitability of human consumption and favourable for fishery.