Background: The sudden outbreak break of crisis made the educational institution to switch over the mode of teaching from traditional method to E-Learning. The main objective of the present study is to assess the existing level of Knowledge, Attitude and Practice about E- learning among B.Sc Nursing Students in selected Nursing colleges, to correlate all the outcome variables with each other. Methodology: A cross sectional descriptive survey study was conducted among B.Sc Nursing Students in selected Nursing colleges, Puducherry with 400 samples, selected through cluster random sampling technique. Quantitative approach was used for this study and the design was descriptive cross-sectional design. Data was collected by using self-structured questionnaire prepared by the researcher. Results: The results revealed that, out of 400 samples, nearly more than half of the samples 211(52.8%) had inadequate Knowledge about E-Learning. Further, the data was analyzed by using the Karl Pearsons correlation and it had shown positive correlation which is found to be statistically significant at p<0.01 level between all three variables. Regarding the association, previous experience of attending classes online classes, gender and total family income per annum had shown significant association with Knowledge, educational status of the mother, total family income per annum and sibling having separate gadgets for attending E-Learning had shown statistically significant association with Attitude, use of gadgets by the subjects siblings having separately for attending E-Learning had statistically significant association with Practice at p<0.05 level. Conclusion: The study concludes that the E-Learning should be recommended for the Students to update their Knowledge and practice with desirable attitude changes to adapt E-learning for their learning platform.