Extensive literature abounds that applauds the importance of fish and soybean oils to human functioning. Evidence suggests that fish and soybean oil constitute a significant public and medical importance to human existence. Numerous authors have emphasized the health benefits of oils. The current research aimed to produce and analyze the nutritional and bioactive constituents of fish and soybean oil widely used in Nigeria. The samples were extracted from fish and soybean seeds using the standard procedures described in the literature. Thirty liters of the oils were removed from the samples, respectively. The nutritional contents and other properties were determined. The study provided insight into the nutritional composition of fish oil and soybean oil. The findings and recommendations are discussed.