A simple, economic, selective, precise, and accurate High Performance liquid Chromatographic method for the analysis of Nitrofuratoin in bulk drug and pharmaceutical formulations was developed and validated in the present study. The mobile phase consists of a mixture of Acetonitrile and buffer 88:12. And adjust the pH to 5.0 ± 0.05 with sodium hydroxide solution. This was found to give a sharp peak of Nitrofuratoin at a retention time of 8.423 min. HPLC analysis of Nitrofuratoin was carried out at a wavelength of 254 nm with a flow rate of 1.6 mL/min. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient of 0.999 in the concentration range of 50 to 150 µg ml-1. The linear regression equation was y =53.4120x-168.66. The developed method was employed with a high degree of precision and accuracy for the analysis of Nitrofuratoin. The developed method was validated for accuracy, precision, robustness, detection and quantification limits as per the ICH guidelines. The wide linearity range, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is better for the quantification of Nitrofuratoin.