Operculina turpethum is a threatened medicinal plant of Convolvulaceae family. It is a potent herb having wide pharmacological actions and therapeutic significance. But, propagation through seed suffers from problems like poor seed viability, low percentage of germination, survivability and dormancy. Hence, there is a need to standardize an alternative method of propagation for the production of true to type plants. In-vitro propagation is one such tool which helps in large scale multiplication of this species.