This report provides methodological briefs that provide short, step-by-step, guidance and lessons learnt on applying key methods used in the H2020 UNISECO project. The briefs are available in electronic form and can be accessed via the Agro-ecological Knowledge Hub as a part of information content for the different target audiences. UNISECO is a European research project aiming to develop innovative approaches to enhance the understanding of socio-economic and policy drivers and barriers for further development and implementation of agro-ecological practices in EU farming systems. Learn more about the project: https://uniseco-project.eu/ This project has received funding from the European Union's H2020 research and innovation programme under grant agreement No 773901. This publication reflects only the authors' view and the European Commission is not responsible for any use that may be made of the information it contains.