Background: The kidney, ureters, bladder, and urethra constitute the urinary system. UTI is an infection of the urinary tract caused by bacteria such as Escherichia coli, Staphylococcus aureus, Klebsiella pneumonia and some others. Urinary tract infections (UTIs) are the second most common type of infection in the body. Objectives: To assess the knowledge, attitude and practice about UTI in adolescent girls, to educate girls on UTI and to assess the impact of education by post questionnaires. Methodology: The study was a prospective, interventional study that lasted six months and was divided into three parts, with some inclusion and exclusion criteria. Pre- test and post-test questionnaires were used to collect data from adolescent girls at selected PU colleges in Tumkur, and students were educated by utilizing PIL and power point presentations. Result: The aggregate number of students who participated in the study was 406, consisting 119 by a Private PU College for Women and 287 from a Government PU College for Women. The participants' mean pre-test score was 52.74 %, which increased to 75.55 % in the post-test. The pre-test score of knowledge, attitude and practice seem to have been 44.02 %, 61.57 %, and 68.41 %, and the post-test scores which was increased to 71.15 %, 91.13 %, and 87.8 % respectively. Conclusion: The study shows there is improvement in basic aspects of the topic after educating. Hence educating adolescents on various infections and encouraging them to modify their life style plays a vital role to lead healthy life.