The paleotropics harbor many biodiversity hotspots and show many different species richness patterns. However, it remains unclear which factors are the most important in directly shaping richness patterns among regions in the paleotropics (i.e. diversification rates, colonization times, dispersal frequency). Here we used Cycadaceae as a model system to test the causes of regional richness patterns in the paleotropics. Specifically we tested the roles of dispersal frequency, colonization time, diversification rates, and their combined role in explaining richness patterns among regions. We generated a well-sampled, time-calibrated phylogeny and then used this to estimate dispersal events, colonization times, and diversification rates. Richness patterns were significantly associated with the timing of the first colonization of each region, and were best explained by the combined effects of colonization time and diversification rates. The number of dispersal events into each region and the diversification rates of species in each region were not significantly related to richness. Ancestral-area reconstructions showed frequent migrations across Wallace's Line, with a higher diversification rate east of Wallace's Line than west of it. Overall, our study shows that colonization time can be an important factor for explaining regional richness patterns in the paleotropics.

The readme file contains an explanation of each file used in this work. Information on how the analyses were done can be found in the associated manuscript referenced above. Funding provided by: National Natural Science Foundation of ChinaCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100001809Award Number: 31900184Funding provided by: Natural Science Foundation of Yunnan ProvinceCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100005273Award Number: 202001AT070072Funding provided by: China Scholarship CouncilCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100004543Award Number: 201804910257Funding provided by: West Light Foundation of the Chinese Academy of SciencesCrossref Funder Registry ID: http://dx.doi.org/10.13039/501100013494Award Number: Y8246811W1