The COVID-19 pandemic has restricted all educational institutions from the traditional campus-based education system to e-learning worldwide. However, adapting to this new platform, e-learning readiness may cause perceived stress among students. This study aimed to examine the association between e-learning readiness and perceived stress as well as the relationship with socio-demographic and e-learning related factors. This cross-sectional study was employed, where 1145 e-learning enrolled university students were surveyed.