The study investigated utilization of science equipment and students’ self-efficacy in Basic Science in Ekiti State Secondary Schools. The descriptive research design of the survey type was adopted in the study. The population of this study consisted of all the teachers and students of all secondary schools in Ekiti State. The sample for this study consisted of 450 students and 30 teachers which were selected from 30 secondary schools in Ekiti State. The sample was selected through multi stage sampling procedure. Two research instruments were used to collect relevant data for the study. These were Basic Science Equipment Checklist (BSEC) and Students’ Self-Efficacy Scale (SSES). The face and content validity of each instrument were done by experts in Tests and Measurement and Science Education to determine the level of appropriateness of the instruments. The reliability of the instruments was established through the test re-test method. The scores of the two tests (i.e. test and re-test) were thereafter correlated using Pearson’s Product Moment Correlation. Reliability Coefficient 0.79 and 0.83 were obtained for BSEC and SSES respectively. The researcher personally administered the two instruments. The data collected for this study were analyzed using descriptive and inferential statistics. The findings of this study revealed that the level of students’ self-efficacy in Basic Science in public secondary schools in Ekiti State is moderate. Furthermore, availability, adequacy and extent of utilization of science equipment have no influence on students’ self-efficacy in Basic Science. It was recommended among others that Basic Science teachers should make requisitions for equipment for teaching and ensure proper utilization. Keywords: Utilization, Science Equipment, Self-Efficacy, Basic Science,