Release Description Fourth major release of AncientMetagenomeDir Release v21.06 includes 10 new publications, representing 142 new ancient host-associated metagenome samples, 33 new ancient microbial genomes and 11 new ancient environmental samples. This brings the repository to a total of 916 ancient host-associated metagenome samples, 355 ancient microbial genomes, and 363 ancient environmental samples. Corrections were made to 1 publications. The full changelog is below. Added Ancient Metagenome: Host Associated Wibowo 2021 10.1038/s41586-021-03532-0 Fellows Yates 2021 10.1073/pnas.2021655118 Farrer 2021 10.1038/s41598-021-86100-w Ancient Single Genome: Host Associated Seguin-Orlando 2021 10.1016/j.isci.2021.102383 Haller 2021 10.1016/j.isci.2021.102419 Guellil 2020 10.1073/pnas.2009677117 Danneels 2021 10.1016/j.cub.2021.03.049 Ancient Metagenome: Environmental Wibowo 2021 10.1038/s41586-021-03532-0 Schulte 2021 10.1111/1755-0998.13311 Lammers 2021 10.1038/s42003-021-01710-4 Added new feature (sea coast) Liang 2021 10.1186/s40168-021-01057-2 Ancient Metagenome: Anthropogenic Misc Changed Changed collection_date column to sampling_date to clarify this is when drilling, or sub-sampling for DNA analysis was performed Corrected date for GLZ001 from Yu et al. (2020) from 4400 to 4600 based on the re-calibrated reported date in the paper (doi: 10.1016/j.cell.2020.04.037) Removed Prehistoric Rock Art Sites in the Côa Valley and Siega Verde The two Prehistoric Rock Art Sites in the Côa Valley (Portugal) and Siega Verde (Spain) are located on the banks of the rivers Agueda and Côa, tributaries of the river Douro, documenting continuous human occupation from the end of the Paleolithic Age. Hundreds of panels with thousands of animal figures (5,000 in Foz Côa and around 440 in Siega Verde) were carved over several millennia, representing the most remarkable open-air ensemble of Paleolithic art on the Iberian Peninsula. Description is available under license CC-BY-SA IGO 3.0 from UNESCO