ENBIOS (Environmental and Bioeconomic System Analysis) is an assessment framework designed for the assessment of the environmental impacts and resource use of energy pathways resulting from energy system optimization models (ESOMs). It integrates Life Cycle Assessment (LCA) and Social Metabolism Assessment using the Multi-Scale Integrated Assessment of Socio-Ecosystem Metabolism (MuSIASEM). It has been been co-designed with decision makers and energy modelers within the EU project SENTINEL. The related python package enbios takes data on energy system design and impact assessment methods to return a characterization matrix filled with bioeconomic and environmental indicators. The deliverable includes a brief introduction to the framework and the python package, the first version of the quick start guide and the user manual.