This dataset aims to test the algorithms presented in an article submitted by the authors and untitled: The combination of chemical reporter-, segmentation- and ratiometric-methods enables high-quality mapping of lignification dynamics in plant cell walls Are available: -the algorithm with graphical user interface for imageJ and its installation procedure ("Cell_Wall_Segmentation " and "Tutorial Cell_Wall_Segmentation") -a folder comprising a classifier and a data set compatible with the machine learning part of the algorithm "data and classifier for weka" - representative images adapted for testing “representative images” - the macro corresponding to the parametric segmentation procedure (see imageJ documentation for installation instructions) “parametric_segmentation”