One hundred and twenty (120) 16 weeks old White Leghorn layer breeds were used for the study. The objective of the study was to determine effect of photoperiod on layer chicken. The research was carried out at the Poultry Section of the Animal farm of the Department of Animal Science Education, Akenten Appiah-Menka University of Skills Training and Entrepreneurial Development, Mampong. Four treatments made up of 12 hours of light, 14 hours of light, 16 hours of light and 18 hours of light were used for the study. Each treatment was replicated three times with 10 birds per replicate in a Completely Randomized Design. Birds were exposed equally to common daylight and in the evenings, lights were turned on at specified periods. Growth parameters measured were initial body weight, daily feed intake, daily weight gain and final body weight. Egg traits measured were egg weight, albumen height, yolk color and yolk weight. The data collected were analyzed using General Linear Model procedure of Statistical Analysis System. Results from the study indicated that, varied photoperiod regimes had no significant (P>0.05) effect on initial body weight, daily feed intake but had significant (P<0.05) effect on daily weight gain and final body weight of growth traits. Photoperiod had no significant (P>0.05) effect on the egg characteristics of the white Leghorn layer bird. It was concluded from the study that, increasing photoperiod had no positive effect on growth and egg characteristics of the white Leghorn layer.