Dataset for: Brenn-White M, Raphael BL, Rakotoarisoa NAT, Deem SL. 2022. Hematology and biochemistry of critically endangered radiated tortoises (Astrochelys radiata): reference intervals in previously confiscated subadults and variability based on common techniques. PLOS One. Dataset is contained in a.radiata_bloodwork.csv. Metadata and data definitions are contained in a.radiate_bloodwork_readme.txt. Dataset contains hematology and biochemistry values of 120 previously confiscated, clinically healthy subadult radiated tortoises living under human care within their native habitat at the Tortoise Conservation Center (TCC), Madagascar. To evaluate the effects of different commonly used techniques on these parameters, we compared results between two venipuncture sites (subcarapacial sinus and brachial vein) and three different WBC quantification methods (Natt and Herrick, LeukopetTM, and slide estimate). Data from tortoises removed from the final analyses due to sample quality issues are also included as frequency of sample quality issues varied by venipuncture site. See associated publication for detailed methods.