Background & objectives: The present investigation was made to find out the nutritional status, serum iron level, Haemoglobin (Hb) concentration and anthropometric characteristics among the pre-menopausal and post-menopausal women. It was also aimed to study the interrelationships among the parameters. Methods: A total of 140 women subjects having the age range of 30-60 years were selected at random for the study. The subjects were further classified into pre-menopausal group (30- 40 years) and post-menopausal group (50- 60 years). Serum iron level and Hb concentration were measured by using biochemical method and anthropometric parameters were measured by using standard techniques. Nutritional status was taken from 24 hours dietary recall method. Results: The results showed that there was a significant difference (**p<0.001) of serum iron levels, Hb concentration and nutritional status between pre-menopausal and post-menopausal women. The higher percentage of pre-menopausal women belongs to normal category, whereas in post-menopausal women belong to overweight category. Energy, carbohydrate and protein intake was significantly correlated (**p<0.001, *p<0.01) with serum iron level and Hb concentration. The post-menopausal women reported a higher percentage of irritability (75%), arthritis (60%), aching of joints and muscles (70%). Interpretation & conclusion: In the present study, we found that the post-menopausal women were suffering most from some of the health and symptomatic problems compared to the pre-menopausal women due to lack of awareness. Hence, an intensive orientation and education is absolutely necessary.