Introduction: Obestatin is a recently discovered peptide produced in the stomach, which was originally described to suppress food intake and decrease body weight in experimental animals. Obesity is an obsessive condition, which results from an irregularity between caloric admission and consumption, and is described by excessive muscle to fat ratio. Aims of the study: The main aim of the present study is to analyze the role of plasma obestatin levels in obesity which is associated with daily life style. Methodology of the study: This study was conducted according to the rules and regulations of ethical committee of the hospital. The data was collected from 50 obese patients which was also suffering from heart and cholesterol diseases. These patients who come at Allied Hospital Faisalabad were selected for this study during Jan 2018 to May 2019. Demographic factors were also asked to the student. Body mass index (BMI) and waist circumference (WC) were done for patients and controls as anthropometrical tests, while fasting serum glucose (FSG) measured using spectrophotometric technique. Each serum sample was analyzed for obestatin hormone and fasting insulin using enzyme linked immune sorbent assay (ELISA). Results: Mean fasting obestatin levels was 0.450 ± 0.468 and 0.959 ± 0.889 separately in hypertensive and normotensive fat and the distinction of mean fasting obestatin levels between the two gatherings was factually huge with p esteem 0.000. Mean fasting blood cholesterol level was 206.42 ± 44.420 and 202.39 ± 48.344 respectively in normal and obese and the difference was not statistically significant with p value 0.644. Conclusion: Obestatin plays very important role in obesity and it is directly correlated with blood glucose level. Furthermore, there was a clear relationship between obestatin and both BP and HOMA-IR, suggesting that obestatin might play a role in BP regulation. Key words: Obestatin, obesity, diseases, patients.