Background:Vitamin D is necessary for calcium absorption and bone mineralization .Too little vitamin D results in soft bones in children (rickets) and fragile, misshapen bones in adults (osteomalacia).Vitamin D deficiency is one of the leading health related problem of the world.Vitamin D is also known as sunlight vitamin so its level in body largely affected by lifestyle and sunlight exposure.Vitamin D status can be assessed by measuring concentrations of 25-hydroxyvitamin D (25(OH)D). Aims and Objectives : This is a comparative study aimed to find out status of vitamin D level among rural and Urban population. Methodology: Study Design and Sampling:It was a cross sectional study.The sampling technique used was non probability purposive sampling. Study Setting and Duration: The study was conducted in BHU Kotli Said Amir and Allama Iqbal Memorial Hospital Sialkot from Jan 2019-Dec 2019. Inclusion and Exclusion Criteria : All patients visited general opd having age above 18years were included except patients with complain of bone pain ,joint pain ,chronic illness and those taking Vitamin D supplementation. Data Collection and analysis :The sample was selected using non probability purposive sampling ,selected sample Vitamin D levels were checked at the time of hospital visit. Result:The study included 2000 patients of BHU Kotli Said Amir and Allama Iqbal Memorial Hospital Sialkot out of which 1000 patients were from rural areas and 1000 patients were from urban areas. The age of sample ranged from 18 years to 88 years with a mean age of 56.Sixty four percent (1280) were females and 36%(720) were males .Sixty eight percent patients (1360 patients) had vitamin D level below 30ng/ml among them 802 patients(40%) are from the urban population and 558 patients(28%) from the rural population . Conclusion: It is concluded from the research that Vitamin D deficiency is highly prevalent in the Urban population ,so awareness programs regarding Vitamin D supplementation and fortification of food with vitamin D should be conducted.