Nigella sativa has been widely used in traditional Turkish medicine for several treatments, specially wounds and diabet disorders. However, the effects of this plant essential oil on wound healing have not yet been clearly explained. Thus study is required to develop new and effective treatment methods to deal with this subject. This present study was focused on utilization of Nigella sativa L. essential oil (NSE) on topical agent for diabetic wound treatment. A total of 72, Sprague–Dawley male rats were used in the present study. The rats were divided into nine groups (n=8). Streptozotocin (STZ) was given at single dose of 60 mg/kg/i.p. The animals showing diabetes (Blood glucose level >250 mg/dL) will be selected for wound groups. Wounds were created by punch (5 mm on dorsal region of each rat). On the 3th, 10th and 14th post-wounding day, the rats were sacrificed and dissected wound tissues. The results of this study showed that lipid peroxidation and oxidative stress significantly increased after STZ application. MDA and GSH levels, GPx, SOD and CAT activities were measured in plasma and wound tissues of the diabetic and treatment groups. While GSH, GPx, SOD and CAT levels in the plasma and wound tissues of the rats were decreased while MDA level was increased compared to the control group in the diabetes group. GSH, GPx, SOD and CAT levels were increased in the Nigella sativa L. essential oil treatments compared to the diabetes group while MDA level was decreased. All changes in biochemical parameters were directly proportionaled with histopathological changes of the wound tissues. NSE can be a play role of reducing of the lipid peroxidation, oxidative stress and associated complications and plays a beneficial role in the treatment of diabetic wound.