A. E. Delorme, J.-M. Andanson and V. Verney: Improving Laccase Thermostability with aqueous Natural Deep Eutectic Solvents, Int. J. Biol. Macromol., (2020), doi.org/10.1016/j.ijbiomac.2020.07.022 Abstract The wide-spread use of laccases in industry is often limited due to the enzyme inactivation over time at conditions which exceeds the operating conditions of the enzymes, which are neutral pH and ambient temperatures (30-40 °C). Today, the most common strategies used to improve enzyme stability are chemical modifications and immobilization of enzymes on solid supports. Although, these techniques have shown promise in improving enzyme stability, they are often synthetically demanding and unsustainable in terms of costs and synthesis route. Deep Eutectic Solvents (DESs) have attracted considerable attention as reaction media in biocatalysis due to their promising compatibility with enzymes and sustainable derivation. In this contribution we demonstrate the possibility of applying DESs as incubation media to inhibit thermal inactivation of laccase T. Versicolor. For example we show that by incubating laccase in 25 wt% of a betaine-xylitol based DES at 70 °C for 15 minutes, the measured residual activity of laccase is a near 10 fold greater than the measured residual activity of laccase when incubated without the DES. The drastic enhancement of the enzyme thermostability by pre-incubation of laccase in DES media showcases a facile, cheap and green method of boosting the stability laccase. Dataset This dataset contains all the UV-kinetic raw data used to calculate the laccase activity in the article “Improving Laccase Thermostability with aqueous Natural Deep Eutectic Solvents”. Data are available in a compressed .zip file with 1 folder (Laccase-thermostability-DES_v1.0_TER_WP4_D4-2) containing 3 files: one tabular file saved in .xlsx format containing all UV-kinetic raw data used to calculate the relative and residual laccase activities for figure 1-6 in the article (doi.org/10.1016/j.ijbiomac.2020.07.022). The Laccase-thermostability-DES_v1.0_TER_WP4_D4-2.xlsx file contains the UV kinetic absorption spectra (at wavelength 417 nm) and each row in represent one spectrum. The spectra are grouped under laccase incubation temperature and length of time of incubation. For each incubation time, three solutions were prepared which signifies the three trials under each incubation times. Each sheet in the .xlsx file represent the data set collected for each laccase incubation medium The Materials_and_experimental_method-D4-2-Laccase-Thermostability-DES.pdf file details the experimental method and conditions for the data acquisition presented in the Laccase-thermostability-DES_v1.0_TER_WP4_D4-2.xlsx. Guidance is also provided on how to use the data to calculate the laccase activity and thermostability. The_metadata_information-D4-2-Laccase-Thermostability-DES.pdf includes more detailed metadata information for the dataset represented in the excel file Laccase-thermostability-DES_v1.0_TER_WP4_D4-2.xlsx.