This work was designed to study the present flora and biodiversity of selected districts of Sabratha region. The study has been carried out in the period between December 2018, and June 2020. A total number of 242 species belonging to 160 genera, and 42 families were collected. Five families are belonging to Monocotyledons and 37 are belonging to Dicotyledons. Analysis of the flora in ration to the number of species and genera belonging to each family showed that the family Asteraceae is the most represented in the study area, followed by Fabaceae then Poaceae. The study has also shown the dominance of the genera Euphorbia and Medicago with 5 species each. Floristic analysis of the collected plant species showed that the Mediterranean plants are the dominant with a number of 94 species, followed by Mediterranean / Iranu – Turanean species with a number of 53 species, while other chorological types were poorly represented. This analysis also showed the dominance of Therophytes with a number of 163 species, followed by Hemicryptophytes with 49 species.