The main objective of the present study was to characterize the phytochemicals from the stem of Amaranthus hybridus. The shade dried stem were powdered and subjected to Soxhlet extaction with n-hexane. The extract obtained was characterized by Gas chromatography?Mass spectrometry. Results revealed that different types of high and low molecular weight chemical entities with varying quantities present in the extracts. The most prevailing compounds were identified as Glycerol 1-myristate, Heneicosane, Cyclohexadecane, Hexadecane, Malonic acid, 3-methylpentyl undecyl ester 2-Methylhexacosane, Eicosane, -(+)-Ascorbic acid 2,6-dihexadecanoate, Octacosane trans-13-Octadecenoic acid, 9,12-Octadecadienoic acid methyl ester, and Palmitic acid. These chemical compounds are considered biologically and pharmacologically important. It is concluded that the hexane extract of Amaranthus hybridus stem possess medicinal value as well as pharmacological activities.