Indole acetic acid (IAA) production is a major property of rhizosphere bacteria that stimulate and facilitate plant growth. The present work deals with isolation, characterization and identification of Indole acetic acid producing bacteria from the rhizospheric soil from Raigad district. Out of ten isolates four isolate were selected as efficient Indole acetic acid producers. Optimization of Indole acetic acid production was carried out at different cultural conditions of pH and temperature with varying media components such as carbon and nitrogen source, tryptophan concentration. Partial purification of IAA was done and purity was confirmed with thin layer chromatography. Subsequently, effect on plant growth was tested by measuring root and shoot length. In conclusion the study suggests the IAA producing bacteria can be used to promote plant growth.