This cross-sectional study was conducted in private dental college undergraduate students and house surgeons of Karachi Pakistan. The duration of study was six months and Convenience sampling technique was used. The close ended questionnaire were distributed and filled by these study participants including third year, final year students and trainee house surgeons. The management of biomedical waste is one of the biggest challenges of the present day regarding human health. Regarding the awareness program 10-25% health care waste can cause severe health issues and the purpose of this study was to find out the knowledge, attitude and practice regarding biomedical waste management regarding undergraduate dental students and house officers. A total of 150 BDS students, third and final year as well as house surgeons were included, 55 students from 3rd year, 68 students from final year and 27 house surgeon (1.81 mean, 2.459 std. deviation) that this included 88 male and 66 female participants (mean of 1.44 and std. deviation of 0.498). The 60.7% person?s had a good response about the biomedical waste should be segregated into different categories too most of the respondents gave no answer about the segregation of waste management is 39.3%. The 53.3% participants concerning the biomedical waste management should fulfilled a part of curriculum at undergraduate level; At the end of this study showed that information and awareness level regarding BMWM of study participant was not sufficient and there was a significant difference in practice and management about the biomedical waste management amongst different studies.