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This is the supplementary material for our publication "General Principles for Yield Optimization of Nucleoside Phosphorylase-Catalyzed Transglycosylations". The .pdf file contains the supplementary information: Author Contributions, Conflict of Interest, Sample analysis by HPLC, Table S1 and S1, Figure S1 and a suggested workflow for NPase-catalyzed nucleoside synthesis. The .xlsx file contains an implementation of the simplified formula for yield prediction (equation (4) ), using previously reported thermodynamic data (10.5281/zenodo.3459298). The Python code and all data calculated from numerical solutions of the system of equilibrium constraints is available elsewhere (10.5281/zenodo.3522588).
This work was supported by the German Research Foundation (DFG) under Germany´s Excellence Strategy – EXC 2008/1– 390540038 (UniSysCat), and by the Einstein Foundation Berlin (ESB) - Einstein Center of Catalysis (EC2) and the Deutsche Forschungsgemeinschaft (DFG), grant number 392246628.
equilibrium constant, nucleoside transglycosylation, nucleoside phosphorylase, nucleosides
equilibrium constant, nucleoside transglycosylation, nucleoside phosphorylase, nucleosides
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