Objectives: Advanced Cardiac Life Support (ACLS) is essential to ensure delivery of optimum medical care in emergency situations. This study is aiming to measure the knowledge, attitudes and experience of ACLS among the students of Majmaah University in Saudi Arabia. Methods: The study prospective is cross-sectional took place between February and August 2019. The study used a questionnaire as a tool to collect opinions of 300 students, the answers were collected and analyzed using SPSS software. Results: Almost half of study sample have poor knowledge of ACLS practice although many have attended BLS (Basic Life Support) courses or workshops. Conclusion: The study revealed that despite having positive attitude training, students fails to answer some of very simple question, which shows their inadequacy in BLS/CPR training. It also indicates that the overall knowledge of BLS/CPR training among Majmah University students is not adequate and need significant improvements in order to save lives.