The application materials in the form of textbooks are still the most basic requirement in the process of learning Biology. Biology is one of the sciences that has the characteristics of the scope of physiological processes and mechanisms in it so it is necessary to use images. Textbook-based on process image in biology learning requires analysis and evaluation skills called Higher Order Thinking Skills (HOTS). This study aimed to examine the effectiveness of the use of textbooks based on process images in the excretion system material process towards students\' higher order thinking skills. The design applied for this research is Pretest-posttest design only, with two sessions in three different classes. Data analysis through the calculation of the value of higher order thinking skill (HOTS). Then, the values were analyzed using the Recognition method to find out the categories of higher-order thinking skills of students. The results of this study indicated three schools included in the categories both with consecutive values, 81.5 (Class A); 80.3 (Class B) and 80.5 (Class C).