As a family, Polygonaceae has a complex taxonomic historical position that experienced different treatmentin the floras. In order to clear delimitation of its tribes in Iran, in this study we consider the macro and micromorphologicalfeatures of tepal epidermis, dorsal leaf epidermis, achene surface, and pollen grains in 10populations from different habitats in Iran. The results of cluster analysis have revealed that Rumiceae has aninteresting position in phenogram topology. Micro-morphological characters proved more efficient in theseparation of Persicarieae elements in Iran than in two other tribes. The results of this project show distinctlyPolygonum s. str. and Persicaria as separate genera in the flora of Iran.