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HISTOLOGICAL AND HISTOMETRICAL FEATURES OF THE INTESTINAL MORPHOLOGY OF BROILER CHICKENS TREATED WITH DIFFERENT DRUG FORMULATION, CONTAINING OREGANO OIL

Authors: Ralitsa Bankova; Dimitrov, Dimitar; Dimitrichka Dimitrova;

HISTOLOGICAL AND HISTOMETRICAL FEATURES OF THE INTESTINAL MORPHOLOGY OF BROILER CHICKENS TREATED WITH DIFFERENT DRUG FORMULATION, CONTAINING OREGANO OIL

Abstract

{"references": ["Apparecida Da Silva, De Sousa Ressotti M. B. M., Zanini F. S., Colnago L. G., Rodriges A. R. M., De Carvalho Nunes L., Zanini S. M., Martins F. V. I. (2009). Intestinal mucosa structure of broiler chickens infected experimentally with Eimeria t\u0435nella and treated with essential oil of oregano. Ciencia Rugal de Santa Maria 2009: Vol. 39(5): 1471\u20131477.", "\u0410vtandilov G. G. (1990). Medical morphometry. Medicine, Moskow, pp. 191\u2013247.", "Betancourt L., Ariza J. C., Afanador G. (2012). Effects of suplementation with oregano essential oil on ileal digestibility, intestinal histomorphology, and performance of broiler chickens. Revista Columbiana de Ciencias Pecuarias: Vol. 25: 240\u2013251.", "Franseca-Garsia I., Escalera-Valente F., Martinez-Gonzales S., Carmona-Gasca A. C., Gutierez Arenas A. D., Aliva-Ramos F. (2017). Effect of oregano oil dietary suplementation on production parameters, high of intestinal villi and the antioxidant capacity in the breast of broiler. Australian Journal of Veterinary Science: Vol. 49: 83\u201389", "Georgescu B., Ciobotaru E., Predoi G., Cormila N. (2007). Research concerning histostructure of cecal tonsils in some species of domestic birds. Lucrari Stiintifice Medicina Veterinaria: XL: 397\u2013 404.", "Giannenas I., Tzora A., Sarakatsianos I., Karamautsios A., Skounfos S., Papaioannous N., Anastasiou I., Skoufos I. (2016). The effectiveness of the use of oregano and laurel essential oils in chickens feeding. Annales of Animal Science: Vol. 16 (3): 779\u2013796", "He X., Hao D., Liu C., Zhang X, Xu D., Wang J., Wu R. (2017). Effect of supplemental oregano essential oils in diets on production performance and relatively intestinal parameters of lying hens. American Journal of Molecular Biology: Vol. 7: 73\u201385.", "Karadas F., Pirgozliev V., Dimitrov D., Rose P., Bravo D. (2012). Dietary plant extracts improve growth and antioxidative status of chickens reared on previously used litters. British Poultry Abstracts: Vol. 8 (1): 39\u201340.", "Karadas F., Beccaccia A., Pirgozliev V., Rose P. S., Dimitrov D., Bravo D. (2013). Dietary essential oils improve hepatic vitamin E concentrations of chickens reared on recycled litter. British Poultry Abstracts: Vol. 9: 31\u201332.", "Karadas F., Pirgozliev V., Rose P. S., Dimitrov D., Oduguwa O., Bravo D. (2014). Dietary essential oils improve the hepatic antioxidative status of broiler chickens. British Poultry Science: Vol. 55(3): 329\u2013334.", "Rezaian M., Hamedi S. (2017). Histological study of the caecal tonsils in the cecum of 4\u20136 mounths of age white leghorn chickens. Journal of Animal and Veterinary Sciences: Vol. 2 (2): 50\u201354.", "Sarica S., Suichmez M., Gorduk M., Ozdemir D., Berberoglu E. (2014). Effects of oregano essential oil suplementation to diets to broiler chicks with delayed feeding after hatching. Morphological development of small intestinal segments. Italian Journal of Animal Science: Vol. 13(3172): 284\u2013 288.", "Tzora A., Giannenas I., Karamoutsios A., Papaioannou N., Papanastasious D., Bonos E., Skoufos S., Bartzanas T., Skoufos I. (2017). Effect of oregano, atapulgite, benzoic acid and their blend on chicken performance, intestinal microbiology and intestinal morphology. Journal of Poultry Science: Vol. 54: 218\u2013227.", "Zou Yi., Xiang Q., Wang J., Peng J., Wei H. (2016). Oregano essential oil improves intestinal morphology and expression of tight junction proteins associated with modulation of selected intestinal bacteria and immune status in a pig model. Hindawi Publishing Corporation Bio Med Research International: ID5436738: 1\u201311."]}

The study was conducted with 40 clinically healthy (equal in both sexes) broiler chickens. Biosamples were taken at the end of the finishing period (day 41) in each group, from each bird, from all parts of the intestinal tract. Durable histological preparations were made by classical methods with each biosample. In the histological study, it was found that there was a microstructured change in all segments of the intestinal mucosa in the two experimental groups – first one, which received Ecodiar® liquid 5% to the drinking water, in dosage 0.5 ml of drug formulation/L water, and second one, which received Ecodiar® powder 5% to the feed, in dosage 0.5 g of drug formulation/kg of feed. In the third experimental group, which received 5 ml of 1% oregano oil/kg of feed and in the fourth (control) group, no histological changes in the intestinal wall were found. In the histometric study, the highest intestinal villi were found in the ileum, followed by the duodenum and the jejunum. Intestinal crypts had the largest outer diameter in the caecum, followed by the ileum, jejunum and duodenum. These two parameters showed different histometric values in each of the test groups of broiler chickens and each of the intestinal segments examined.

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Keywords

chickens,, oregano oil,, gut, histological study,

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