The genus Acanthistius Gill, 1862 comprises ten putative valid species occurring in shallow warm-temperate waters of the Southern Hemisphere: South America (coasts of Ecuador, Peru, Chile, Brazil, and Argentina) southern Africa (Namibia and South Africa), southern Australia, New Zealand, Lord Howe, Norfolk, Kermadec and Easter, and Sala y Gómez islands. Two species: Acanthistius sebastoides (Castelnau, 1861) and Acanthistius sp are known from shallow waters of the east coast of South Africa (Heemstra and Randall, 1986). The latter taxon, previously known from a brief description of a single specimen, is here described from 23 specimens and named Acanthistius joanae. Acanthistius sebastoides is redescribed from 13 specimens and compared with A. joanae and species of Acanthistius known from South America and Australia. A neotype is designated for Serranus sebastoides Castelnau, 1861, as the two syntypes are apparently lost.