Sucralose (Sc) is a non-nutritive sweetener derived from sucrose. This sweetener has gained great popularity in the food industry. The objective of this work was to analyze the effect of Sc (Splenda) interactions on Nitrogenous bases (NB) (DNA and RNA). We started the investigation with the Sc molecule. The structure was taken from the PubChem page. Then it was designed in the hyperchem simulator. These interactions are studied in two groups. A) As pure substances. We found that Sc has a very high ETC and for that reason is very unstable. Due to this instability of the Sc, it can attack any NB. B) As substances in crossed bands. HOMO of substance one vs. LUMO of substance two. We found interactions of high probability and high stability and chemical affinity between the Sc as an oxidizing agent and the NBs: C, G, and A. These interactions are hazardous for human health.