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The paper describes a case of an acute lethal infection in guinea pigs, demonstrated with watery to bloody profuse diarrhea, respiratory paralysis and death. Microbiological tests indentified several isolates: Clostridium novyi (oedematiens) (C. novyi), Escherichia coli (E. coli), Enterococcus sp. and Bacillus cereus (B. cereus). Materials from internal organs were obtained for pathohistological examination. History data, microbiology and histopathology were evidential for toxin-forming pathogenic microorganisms, imported by food and/or probably turned to virulent endogenous bacterial strains, causing the death of the animals.