The present study was conducted to evaluate the chemical composition, Phenolic, flavonoid content and the antimicrobial activity of methanol extract of Star anise fruit. The chemical composition, minerals and antimicrobial investigations of star anise were analyzed using different standard techniques and HPLC analysis. Total phenols was determined (mg/kg) was 28.59. The total antioxidants 95%. Total. The chemical composition and mineral content of the star anise fruit indicate that the fruit has high carbohydrate and potassium level which can directly add nutrient valve to the food. Phenolic and flavonoids are considered to be non-enzymatic antioxidants with high scavenging potential to the free radical produced from the oxidative stress. Our study indicates that the fruit contains the high antioxidative property so it can be used as the natural antioxidant to protect us from oxidative damage. Keywords: Star anise, Chemical composition, Minerals, HPLC.