There are plants of almost all vegetation types and ecological groups, and from the most part of provinces of 5 floristic regions such as Circumboreal, Iran-Turanium, Mediterranean, Atlantic-North American, and Eastern Asiatic ones, and plants of many life forms in collection of perennial herbs ornamental species of the Donetsk Botanical Gardens. It is revealed that species of the different ecobiomorphes, species of ecological and geographical distribution are characterised by the different level of adaptation to the Ukrainian south-east conditions. Thirty one species of ornamental perennial plants which can be recommended for cultivation in the Ukrainian south-east are chosen. It is established that species of geophyte and hemicrytophyte life form, species of mesophyte and xerophyte ecological groups, species of meadow, forest and steppe cenotic association obtained the highest introduction assessment. Species of cohesive, holarctic and pluriregional geoelement and some endemic species turned out to be the most adapted to the Ukrainian south-east conditions.