The research was aimed to enhance growth, production, and seed quality of Jack Bean through pruning, cropping pattern and spacing regulation. The research was carried out from Mei to October 2016 at Puwasari Village, Dramaga, Bogor – Indonesia and be countinued by seed testing (December 2016) at Seed Testing Laboratoty, Bogor Agricultural University. The research was arranged in a Completely Randomyzed Block Design (CRBD) with 6 treatments, consisted of: Without pruning treatment using square spacing pattern and spacing 100cm x 100cm, Without pruning treatment using square spacing pattern and spacing 70cm x 70cm, Without pruning treatment using double row pattern and spacing 50cm x 50cm x 100cm, Pruning treatment using square spacing pattern and spacing 50cm x 50cm, Pruning treatment using square spacing pattern and spacing 70cm x 70cm, Pruning treatment using double row pattern and spacing 50cm x 50cm x 100cm. The experiment was replicated by three replications. The result research showed spacing regulation and pruning has significantly effect to some variables observed. The wider spacing showed the better growth. The treatment of without pruning treatment using square spacing pattern and spacing 70cm x 70cm (P2) resulted better growth, while the treatment of pruning treatment using square spacing and spacing 70cm x 70cm (P5) resulted better seed quality, however the highest production was reached by pruning treatment using double row pattern and spacing 50cm x 50cm x 100cm (P6).