In order to determine the relationship between high school teachers' workplace friendship and school culture perceptions, the research was carried out with a total of 330 teachers from 8 Anatolian high schools and 8 vocational high schools selected by stratified sampling method in Efeler District of Aydin Province, Turkey in the 2017-2018 academic year. When the data were analyzed, 8 data collection tools which were filled in and which did not carry normality conditions were removed without investigation and analyzed through 322 data collection tools. In the collection of the data "Personal Information Form” created by researchers; "Workplace Friendship Scale" that it developed by Nielsen, Jex and Adams (2000), adapted to Turkish by Kiral (2016) and the "School Culture Scale" developed by Terzi (2005) was used. An exploratory and confirmatory factor analysis was conducted for the construct validity of the School Culture Scale. In the analysis of the data, t-test, Kruskal Wallis H-test, ANOVA, Scheffe test were used. The Pearson Moments Multiplication Correlation Coefficient was used to determine the relationship between workplace friendship and school culture perception of high school teachers. According to the results of the research, it was found that the "friendship opportunity" and "task culture" perceptions of the high school teachers were higher than the other dimensions. The friendship prevalence of female teachers is higher than that of male teachers; the friendship prevalence and general friendship of married teachers is higher than that of single teachers; the bureaucratic culture perception level of high school teachers in the age group of 35 and under is higher than 41-45 age group; the achievement culture perception level of vocational high school teachers is higher than that of Anatolian high school teachers. Positive and significant relationship between workplace friendship and school culture was found.