Essential Biodiversity Variables (EBV) are information products for assessing biodiversity change. Species populations EBVs are one class of EBVs that can be used to monitor the spread of invasive species. However, systematic, reliable, repeatable procedures to process primary data into EBVs do not yet exist, and environmental research infrastructures still must improve their capabilities to deliver EBV data products. The authors tested the ability of two mature biodiversity data infrastructures, the Global Biodiversity Information Facility (GBIF) and the Atlas of Living Australia (ALA) to cooperatively produce EBV data products for three alien invasive species. They detailed workflow steps to discover, filter, retrieve and prepare the primary data before evaluating species’ distributional changes. The work is reported in the following journal article: Hardisty, AR., Belbin, L., Hobern, D., McGeoch, MA., Pirzl, R., Williams, KJ., and Kissling, WD. 2018. Research infrastructure challenges in preparing Essential Biodiversity Variables data products for alien invasive species. Environ. Res. Lett. https://doi.org/10.1088/1748-9326/aaf5db This data package accompanies that work and contains the data derived at each main step of an Invasive Species Distribution (IVSD) workflow for a prototype Essential Biodiversity Variable (EBV) data product.