Abstract: The objectives of the study, which was conducted in 2025, were both to explore 344 pre-service teachers’ perceptions and attitudes on Artificial Intelligence (AI) and ways of implementing it in their Teaching Practice, based on the TPACK mod-el, and examine the differences regarding gender and age, through a questionnaire which was designed based on the tool developed by Chounta et al. (2022). According to the results, pre-service teachers at the Department of Pedagogy and Primary Education of the National and Kapodistrian University of Athens (NKUA) seem to be familiar with the concept and have used relevant applications. However, the use and the aim of using it during their Teaching Practice seems to be limited. The results highlight the necessity for preparing future teachers to address educational challenges through the utilization of AI tools.

Submitted: July 2025, Revision Submitted: December 2025, Accepted: February 2026