Dailognatha quadricollis (Coleoptera: Tenebrionidae) is a darkling beetle native to the Balkans and Eastern Mediterranean, with a range extending from Croatia to Lebanon. It is a morphologically diverse species, comprising numerous subspecies, particularly concentrated in the Aegean region. The reference genome of Dailognatha quadricollis will enable phylogenetic, population and evolutionary research. The entirety of the genome sequence was assembled into 11 contiguous chromosomal pseudomolecules and the X sex chromosome. This chromosome-level assembly encompasses 0.52 Gb, composed of 393 contigs and 322 scaffolds, with contig and scaffold N50 values of 4.3 Mb and 23.6 Mb, respectively.